Przemysław Jagodzik

About me

My name is Przemysław Jagodzik, and since 2023 I have been working as a Postdoctoral Research Fellow (Assistant Professor – Post-doc) at the Department of Molecular and Clinical Genetics at the Institute of Human Genetics, Polish Academy of Sciences in Poznań. My research focuses on primary ciliary dyskinesia (PCD), a specific form of ciliopathy resulting from defects in motile cilia. As part of my work, I investigate the OFD1 interactome to identify which OFD1‑interacting proteins contribute specifically to PCD. To address these research questions, I primarily use CRISPR/Cas9 genome editing, co‑immunoprecipitation (Co‑IP), and protein analysis by Western blotting.

Since August 2025, I have also been leading a research project funded by the National Science Centre (MINIATURA 2025/09/X/NZ4/00289). The project investigates the relationship between motile cilia dysfunction and nitric oxide levels, as well as the functioning of the NO–sGC–cGMP–PKG signaling pathway in the context of PCD.

I have a lot of experience in proteomics, which I started gaining since 2009, when I started my master's degree. As part of my master's thesis, I was looking for potential MAPKKK18 kinase protein partners (Arabidopsis thaliana), which are elements of the signaling cascade with its participation. In 2013, I started doctoral studies at the Faculty of Biology of the University of Adam Mickiewicz in Poznań, and the subject of my research was the chaperone activity of the chloroplast protein AtDeg2. I defended my PhD thesis on September 16, 2019. During the preparation of my doctoral dissertation, I mastered many techniques of molecular biology (such as Western blot or diagonal electrophoresis). I was a pioneer in implementing many genetic engineering techniques in my Department, such as: DNA cloning, overexpression and purification of recombinant proteins, plant transformation or protoplast transfection. In addition, I learned the basics of bioinformatics and statistics. The result of my doctoral dissertation is 5 articles published in journals from the Philadelphia list and a series of communications presented at national and international conferences.

In the last years of my PhD I took up a full-time job at the Karol Marcinkowski University of Medical Science in Poznań as a senior scientific and technical worker. During this short period (three months), I learned the basics of cell culture, fluorescence microscopy analysis and ELISA. Then I started working at BioInfoBank Institute in Poznań as investigator in the project aimed in creation of the innovative anticancer pharmaceuticals characterized by high effectivity and selectivity with accompanying minimalized side effects of the foreseen therapy and in the procect aimed in creation an innovative system for DNA removal from biological samples (POIR.01.01.01-00-0975/16). During both projects I learned many new approaches related to cloning, overexpression, purification and biochemical analysis of recombinant proteins.

After my PhD I started working as an Assistant Professor (Post-doc position) at the Department of Plant Ecophysiology of the University of Adam Mickiewicz in Poznań. The main area of ​​my research was the analysis of the role of nitric oxide in the pathogen-plant interaction by the example of potato blight disease caused by Phytoftora infestans (Mont.) De Bary. I analyzed post-translational modifications of proteins such as histone acetylation or methylation and protein nitration or S-nitroslation, and the main techniques I used were Western blot Co-IP and biotin switch method.

To sum up, my experience in the field of proteomics is very broad and covers the area of ​​protein-protein interaction, enzymatic activity and post-translational modifications. As part of the research, both during the preparation of my doctoral dissertation and professional work, I have gained the following skills and professional experience in the field of:

Protein Biochemistry & Proteomics
• Co-immunoprecipitation (Co-IP)
• Diagonal 2D (D2D) SDS-PAGE
• Western blotting (WB)
• ELISA
• Biotin-switch method for S-nitrosylated proteins
• Recombinant protein extraction and purification
• Protein overexpression in E. coli
• FPLC and column chromatography (SEC, HIC, IEX, affinity)
• Yeast two-hybrid (Y2H) assays
• Biochemical assays
Molecular Biology & Genetic Engineering
• CRISPR/Cas9 genome editing in mammalian cells
• DNA cloning (Gateway, restriction enzyme–based)
• Site-directed mutagenesis
• In vitro transcription
• PCR
Microbial, Plant & Eukaryotic Systems
• Transformation of E. coli, A. tumefaciens, S. cerevisiae, A. thaliana
• Isolation and transfection of A. thaliana protoplasts
• Mammalian cell culture and transfection
• Flow cytometry
• Cultivation of Phytophthora infestans
Bioinformatics & Data Analysis
• Perseus software for MS/MS data analysis
• BLAST, ClustalW, MEGA, Jmol, Polyview, Origin

Articles

2025:
• Jagodzik P., Zietkiwicz E., Bukowy-Bieryllo Z..
  Conservation of OFD1 Protein Motifs: Implications for Discovery of Novel Interactors and the OFD1 Function.
  Int. J. Mol. Sci. 2025, 26(3), 1167; https://doi.org/10.3390/ijms26031167. Impact Factor (IF) 2023: 4.9; citations: 0
  29.01.2025
2023:
• Arasimowicz-Jelonek M., Floryszak-Wieczorek J., Suarez S., Doctorovich F., Sobieszczuk-Nowicka E., Bruce King S., Milczarek G., Rębiś T., Gajewska J., Jagodzik P., Żywicki M.
  Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana.
  Nat Plants. 2023 Jan;9(1):36-44. https://doi.org/10.1038/s41477-022-01301-z. Impact Factor (IF) 2023: 15.8; citations: 17
  23.12.2022 (online)
2022:
• Drozda A., Kurpisz B., Guan Y., Arasimowicz-Jelonek M., Plich J.,Jagodzik P., Kuźnicki D., Floryszak-Wieczorek J.
  Insights into the expression of DNA (de)methylation genes responsive to nitric oxide signaling in potato resistance to late blight disease.
  Front Plant Sci. 2022 Dec 2;13:1033699 https://doi.org/10.3389/fpls.2022.1033699. Impact Factor (IF) 2022: 5.6; citations: 4
  02.12.2022
• Arasimowicz-Jelonek M, Jagodzik P, Płóciennik A, Sobieszczuk-Nowicka E, Floryszak-Wieczorek J, Mattoo A, Polcyn W.
  Dynamics of nitration phenomenon during dark-induced leaf senescence in Arabidopsis reveals proteins modified by tryptophan nitration
  J Exp Bot. 2022, erac341 https://doi.org/10.1093/jxb/erac341. Impact Factor (IF) 2022: 6.9; citations: 5
  19.08.2022
• Drozda A, Kurpisz B, Arasimowicz-Jelonek M, Kuźnicki D, Jagodzik P, Guan Y, Floryszak-Wieczorek J.
  Nitric Oxide Implication in Potato Immunity to Phytophthora infestans via Modifications of Histone H3/H4 Methylation Patterns on Defense Genes
  Int. J. Mol. Sci. 2022;23, 4051 https://doi.org/10.3390/ijms23074051. Impact Factor (IF) 2022: 5.6; citations: 5
  6.04.2022
• Jagodzik P, Jackowski G.
  Chloroplast protease/chaperone AtDeg2 holds γ₁ subunit of ATP synthase in an unaggregated state under high irradiance conditions in Arabidopsis thaliana
  Photosynthetica 2022;60 (2): 56-62 https://doi.org/10.32615/ps.2022.004.. Impact Factor (IF) 2022: 2.7; citations: 0
  15.02.2022
2018:
• Jagodzik P, Tajdel-Zielinska M, Ciesla A, Marczak M and Ludwikow A.
  Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling.
  Front. Plant Sci. 2018;9:1387. https://doi.org/10.3389/fpls.2018.01387. Impact Factor (IF) 2018: 4.3; citations: 283
  5.10.2018
• Adamiec A*, Jagodzik P*, Wyka TP, Ludwików A, Mituła F, Misztal L, Luciński R, Jackowski G.
  Chloroplast protease/chaperone AtDeg2 influences cotyledons opening and reproductive development in Arabidopsis.
  Acta Soc Bot Pol. 2018;87(2):3584. https://doi.org/10.5586/asbp.3584. *These authorscontributed equally to this work. Impact Factor (IF) 2018: 0.8; citations: 0
  29.06.2018
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  The contribution of individual domains of chloroplast protein AtDeg2 to its chaperone and proteolytic activities.
  Acta Soc Bot Pol. 2018;87(1):3570. https://doi.org/10.5586/asbp.3570. Impact Factor (IF) 2018: 0.8; citations: 1
  23.02.2018
2014:
• Jagodzik P, Adamiec M, Jackowski G.
  AtDeg2 - a chloroplast protein with dual protease/chaperone activity.
  Acta Soc Bot Pol 83(3):169–174. http://dx.doi.org/10.5586/asbp.2014.018. Impact Factor (IF) 2014: 1.4; citations: 0
  9.07.2014

Conference Reports

2025:
• Jagodzik P, Przystałowska-Macioła H, Dąbrowska M, Bukowy-Bieryllo Z.
  Detection of OFD1 interactions in HEK293 cells across cell cycle stages. 6th BIO Life Science Congress of the Polish Biochemical Society BIO2025.
  Poznan, Poland. 17-20.09.2025
• Kubiak M, Jagodzik P, Bukowy-Bieryllo Z, Pietralik-Molińska Z, Taube M, Chandran N, Kmiecik S, Kozak M, Zietkiwicz E.
  Amino acid substitutions as the molecular basis for primary ciliary dyskinesia (PCD).
  6th BIO Life Science Congress of the Polish Biochemical Society BIO2025.
  Poznan, Poland. 17-20.09.2025
• Jagodzik P, Zietkiwicz E, Bukowy-Bieryllo Z.
  Evolutionary Conservation of Short Linear. Motifs in OFD1: Implications for Discovery of Novel OFD1 Interactors and Function.
  6th BIO Life Science Congress of the Polish Biochemical Society BIO2025.
  Poznan, Poland. 17-20.09.2025
• Jagodzik P, Zietkiwicz E, Bukowy-Bieryllo Z.
  Conservation of OFD1 protein motifs: implications for discovery of novel interactors and the OFD1 function.
  II International ERN-LUNG PCD Meeting in 2025.
  Naples, Italy. 06-08.04.2025. Best Poster Award
2022:
• Gajewska J, Jagodzik P, Kosmala A, Floryszak-Wieczorek J, Arasimowicz-Jelonek M.
  Nitric oxide controls gene expression of Phytophthora infestans by targeting histone deacetylases
  National Scientific Conference on the occasion of the 10th anniversary of the Polish Mycological Society
  Poznan, Poland. 24-28.09.2022
• Jagodzik P, Płóciennik A, Sobieszczuk-Nowicka E, Floryszak-Wieczorek J, Arasimowicz-Jelonek M.
  Identification of nitrotryptophan-containing proteins in Arabidopsis WT leaves undergoing dark-induced senescence
  Redox Biology Congress 2022. Free Radical Biology and Medicine 189:47
  https://doi.org/10.1016/j.freeradbiomed.2022.06.197
  Ghent, Belgium. 23-26.08.2022
• Arasimowicz-Jelonek M, Sobieszczuk-Nowicka E,Jagodzik P, Płóciennik A, Floryszak-Wieczorek J.
  Dynamics of nitration phenomenon during dark-induced leaf senescence of Arabidopsis WT plants
  Redox Biology Congress 2022. Free Radical Biology and Medicine 189:47
  https://doi.org/10.1016/j.freeradbiomed.2022.06.198
  Ghent, Belgium. 23-26.08.2022
• Arasimowicz-Jelonek M, Gajewska J, Rajek M,Jagodzik P, Floryszak-Wieczorek J.
  Pipecolic and azelaic acids confer systemic immunity of potato to Phytophthora infestans by transient nitric oxide generation and histone H3 acetylation
  EPI-CATCH Conference 2022:EPIgenetic mechanisms of Crop Adaptation To Climate cHange
  Crete, Greece. 12-14.07.2022
2021:
• Jagodzik P, Płóciennik A, Sobieszczuk-Nowicka E, Floryszak-Wieczorek J, Arasimowicz-Jelonek M.
  Protein nitrotryptophan formation as a novel NO-dependent modification during dark-induced leaf senescence in Arabidopsis
  Proceedings of the International Scientific Conference, dedicated to the 75th anniversary of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine
  Kyiv, Ukraine. 17.06.2021
2019:
• Jackowski G, Luciński R, Wyka T, Jagodzik P..
  Czy chloroplastowe białko opiekuńcze AtDeg2 uczestniczy w regulacji ultrastruktury chloroplastu i aktywności fotosyntetycznej liści Arabidopsis thaliana?
  58. Zjazd Polskiego Towarzystwa Botanicznego „Botanika bez granic”.
  Kraków, Poland, 1-7.07.2019
2018:
• Jagodzik P, Mituła F, Ludwików A, Jackowski G.
  Chloroplast protease/chaperone AtDeg2 holds γ-subunit of ATP synthase in unaggregated state under high irradiance conditions in Arabidopsis thaliana.
  4th Plant Protease and Programmed Cell Death Symposium.
  Ghent, Belgium, 11-13.09.2018
• Jagodzik P, Mituła F, Ludwików A, Jackowski G.
  Identyfikacja fizjologicznego substratu holdazowej aktywności opiekuńczej chloroplastowej proteazy AtDeg2.
  Konferencja Naukowa Sekcji Fizjologii i Biochemii Roślin PTB oraz Oddziału Lubelskiego PTB "Fotosynteza w świetle badań fizjologicznych i biochemicznych".
  Lublin, Poland, 2.07.2018
• Jagodzik P, Luciński R, Misztal L, Jackowski G
  Chloroplastowa proteaza AtDeg2 posiada aktywność dezagregazy.
  VI Warsztaty Naukowe Instytutu Biologii Ekspreymentalnej Uniwersytetu im. Adama Mickiewicza w Poznaniu.
  Poznań, Poland, 15.06.2018
• Adamiec A, Jagodzik P, Wyka TP, Ludwików A, Mituła F, Misztal L, Luciński R, Jackowski G
  Chloroplastowe białko AtDeg2 uczestniczy w regulacji otwierania się liścieni oraz przebiegu fazy generatywnej u Arabidopsis.
  VI Warsztaty Naukowe Instytutu Biologii Ekspreymentalnej Uniwersytetu im. Adama Mickiewicza w Poznaniu.
  Poznań, Poland, 15.06.2018
2017:
• Jagodzik P, Mituła F, Luciński R, Misztal L, Ludwików A, Jackowski G.
  The search for native protein substrates for chaperone actvity of chloroplast protein AtDeg2.
  8th Conference of the Polish Society of Experimental Plant Biology "Communication in plants: from cell to environment"
  Białystok, Poland, 12-15.09.2017
• Adamiec M, Jagodzik P, Mituła F, Luciński R, Misztal L, Ludwików A, Jackowski G.
  The involvement of chloroplast protein AtDeg2 in chronological progression of ontogenesis stages in Arabidopsis thaliana.
  8th Conference of the Polish Society of Experimental Plant Biology "Communication in plants: from cell to environment"
  Białystok, Poland, 12-15.09.2017
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  Two approaches confirm that chloroplast protease AtDeg2 is able to inhibit aggregation of denatured proteins.
  IV International Conference on Research and Education
  Poznań, Poland, 6-8.04.2017
2016:
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  The importance of a proteolytically active serine and PDZ domains for chaperone activity of chloroplast protease AtDeg2.
  2nd Congress of Polish Biochemistry, Cell biology, Biotechnology and Bioinformatics BIO 2016 “Expanding beyond the limits”.
  Wrocław, Poland, 13-16.09.2016
• Jackowski G, Jagodzik P, Luciński R, Misztal L.
  Two PDZ domains are required for the chaperone activity of chloroplast protease AtDeg2.
  The 17th International Congress on Photosynthesis Research.
  Maastricht, The Netherlands, 7-12.08.2016
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  The involvement of the active site S268 in the protease and chaperone activity of chloroplast protein AtDeg2.
  Plant Biology Europe EPSO/FESPB 2016 Congress.
  Prague, Czech Republic, 26-30.06.2016
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  Udział indywidualnych domen chloroplastowego białka AtDeg2 w jego aktywności opiekuńczej.
  57. Zjazd PTB „Botanika - tradycja i nowoczesność”.
  Lublin, Poland, 26-30.06.2016
• Jagodzik P, Luciński R, Misztal L, Jackowski G.
  The contribution of individual domains of chloroplast protease AtDeg2 to its chaperone activity.
  Plant Proteases: from roles in life and death to understanding regulation and action.
  Oxford, UK, 10-12.04.2016
2014:
• Jagodzik P, Misztal L, Jackowski G.
  Obtainment of Esherichia coli strains overexpressing eight versions of Arabidopsis thaliana chloroplast protease/chaperone AtDeg2.
  7th International Conference of PhD Students of Szczecin University, Biology Panel “Biodiversity at all levels of life”.
  Szczecin, Poland, 17.10.2014
• Jagodzik P, Ludwików A, Jackowski G.
  Introduction of mutated version of AtDEG2 gene into destination vector for studies on functional significance of AtDeg2 chloroplast protease/chaperone.
  1st Congress of the Polish Biochemistry, Cell Biology, Biophysics and Bioinformatics BIO 2014.
  Warszawa, Poland, 9-12.09.2014
• Jagodzik P., Ludwików A., Misztal L., Jackowski G.
  Uzyskanie sześciu wariantów genu kodującego chloroplastową proteazę AtDeg2 i ich wklonowanie do wektora wejściowego.
  3th Scientific-Didactic Conference of the Faculty of Biology of the Adam Mickiewicz University.
  Poznań, Poland, 10-12.04.2014
2011:
• Ludwików A, Kubiak P, Szabat M, Górska A, Jagodzik P, Ziółkowski P, Cieśla A, Czaczyk K, Sadowski J.
  Signal transduction by protein degradation – a new function of protein phosphatases 2C clade A in Arabidopsis.
  FEBS Workshop, Plant Organellar Signaling, From Algae to Higher Plants.
  Primosten, Croatia, 31.08-3.09.2011
• Ludwików A, Piechalak A, Małecka A, Szabat M, Jagodzik P, Górska A, Ziółkowski P, Sadowski J.
  Protein phosphatases: signalling hubs integrating plant stress response
  2th Scientific-Didactic Conference of the Faculty of Biology of the Adam Mickiewicz University.
  Poznań, Poland, 5-7.04.2011